| 报告题目： |
Global regulation mediated by intron retention in immune system |
| 报告人： |
Prof. Weiqun Peng |
| 报告人单位： |
The George Washington University |
| 报告时间： |
10月19日（星期五）上午10点 |
| 报告地点： |
科技楼南511 |
| 报告摘要： |
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My lab focuses on understanding gene regulation using computational genomics and epigenomics approaches. In this talk, I will focus on gene regulation after transcription. In T cell activation process, we observed pronounced discordance between expression changes and epigenomic changes. Meanwhile, we observed that intron retention (IR), a major form of alternative splicing, is prevalent in polyadenylated transcripts in resting CD4+ T cells and is significantly reduced upon T cell activation. These findings lead to the identification of a novel post-transcriptional regulatory mechanism whereby the transcript stability is modulated by regulated intron retention. Furthermore, we perform in-depth analysis of IR across mammalian immune cell-types. Interestingly, in adaptive immune cells (AICs), we find that IR is prevalent in the naïve state and significantly reduced upon activation, with the reduction in IR significantly correlated with gene upregulation. In contrast, IR remains unchanged upon activation in innate immune cells (IICs). This difference in IR behavior between AICs and IICs is highly associated with distinct dynamics in splicing and mRNA degradation pathways. Furthermore, we find that IR dynamics is linked with transcriptional read-through, suggesting that, in AICs, the control over RNA maturation is lax before activation and is tightened up after activation.In summary, our study reveals a strategic difference in global regulation, as mediated by mRNA biogenesis, between the activation process in AICs and IICs. |
| 报告人简介： |
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Prof.Weiqun Peng received his Ph.D. at University of Illinois at Urbana Champaign in 2001 for research on statistical physics and soft condensed matter systems. From 2001 to 2004, he did postdoctoral research at the University of California San Diego. He joined the George Washington University in 2004. He is now a full Professor in the Department of Physics and an Adjunct Professor in the Department of Anatomy and Cell Biology at the George Washington University. His research focuses on understanding how things work in the context of biological systems, using both data-driven and modeling approaches. He has published 40+ papers in scientific journals and received 7,000+ citations. |
